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Image Search Results
Journal: Frontiers in Endocrinology
Article Title: Drosophila Insulin-Like Peptides DILP2 and DILP5 Differentially Stimulate Cell Signaling and Glycogen Phosphorylase to Regulate Longevity
doi: 10.3389/fendo.2018.00245
Figure Lengend Snippet: Glycogen phosphorylase (GlyP) phosphorylation is required for longevity conferred by mutation of dilp2 . Survival calculated from life tables based on estimated Gompertz mortality parameters for UAS-GlyP expression in a dilp2 mutant background with transgenes driven by systemic DaGS-Gal4. Gompertz mortality scale (l, frailty) parameters of transgene expression genotypes were adjusted for the impact of RU alone upon mortality estimated from control genotypes. (A) Overexpression of wild-type GlyP does not extend longevity (Cox hazard analysis, χ 2 = 0.1, p = 0.76), (B) expression of GlyP S15A decreases lifespan (Cox hazard analysis, χ 2 = 24.2, p < 0.0001), and (C) expression of GlyP S15D does not extend longevity (Cox hazard analysis, χ 2 = 1.2, p = 0.28). Each genotype cohort n = 351–361 adults.
Article Snippet: Wild-type, S15A, and S15D GlyP coding sequences were sub-cloned into the
Techniques: Mutagenesis, Expressing, Over Expression